GK073 ScienCell 人類小膠質(zhì)細胞Sensome qPCR檢測試劑盒，Human Microglial Sensome qPCR Array Kit

ScienCell 研究實驗室（ScienCell）是一家生物技術公司，其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞，細胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量，實現(xiàn)細胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥，公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商，為客戶提供，正規(guī)進口，貨期穩(wěn)定，折扣好的服務標準。

GK073 ScienCell人類小膠質(zhì)細胞Sensome qPCR檢測試劑盒，Human Microglial Sensome qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌： ScienCell

產(chǎn)品貨號：GK073

產(chǎn)品名稱：GK073 ScienCell人類小膠質(zhì)細胞Sensome qPCR檢測試劑盒，Human Microglial Sensome qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK073 ScienCell人類小膠質(zhì)細胞Sensome qPCR檢測試劑盒，Human Microglial Sensome qPCR Array Kit， 產(chǎn)品說明：

ScienCell的GeneQuery™人小膠質(zhì)細胞SensomeqPCR陣列（GQH-MGS）有助于對88個關鍵基因進行基因表達分析，這些基因涉及小膠質(zhì)細胞如何感知其環(huán)境。 小膠質(zhì)細胞被認為是“中樞神經(jīng)系統(tǒng)的常駐巨噬細胞"，因此它們?nèi)绾胃兄h(huán)境對于在病原體入侵、損傷或變性期間維持大腦中的穩(wěn)態(tài)功能是bukehuoque的。 該特定陣列中包含的基因側(cè)重于在衰老過程中具有dute差異表達的基因。 如何根據(jù)其功能對所包含的基因進行分組的示例如下所示：

經(jīng)典灌注：PTGS2、TNF、CXCL10

替代引發(fā)：SPP1，馬可，CXCR4，CLEC7A，CD163

趨化因子受體：PTAFR，CMKLR1，GPR77，C5AR1，CCR5

嘌呤能受體：TMEM173，P2RY1，P2RY12，GPR34，ADORA3

老化： TREM2， TLR8， SCARA3， IFITM3， AGER

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可識別并有效擴增特定靶基因的 cDNA。精心設計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Microglial Sensome qPCR Array (GQH-MGS) facilitates gene expression profiling of 88 key genes involved in how microglia perceive their environment.  Microglia are considered to be “the resident macrophages of the CNS," so how they sense their environment is integral to maintaining homeostatic functions in the brain during pathogen invasion, injury, or degeneration.  The genes included in this particular array focus on genes with unique differential expression in aging.  Examples of how included genes may be grouped according to their function are shown below:

·         Classical priming: PTGS2, TNF, CXCL10

·         Alternative priming: SPP1, MARCO, CXCR4, CLEC7A, CD163

·         Chemokine receptors: PTAFR, CMKLR1, GPR77, C5AR1, CCR5

·         Purinergic receptors: TMEM173, P2RY1, P2RY12, GPR34, ADORA3

·         Aging: TREM2, TLR8, SCARA3, IFITM3, AGER

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人小膠質(zhì)細胞森納體 qPCR 陣列板布局*（8 個粗體和斜體對照）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產(chǎn)品訂購信息：

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